Identification of putrefied bodies is a crucial and customary process in forensic routine. Usually, the identification of deceased is completed by visible recognition, fingerprints, bodily distinguishing marks (e.g. tattoos, scars and surgical implants) and/or dental examination. However, if morphologic traits will not be recognizable, because of superior putrefaction of the corpse, or current medical information will not be accessible, the DNA-based identification is favored. Thus, in order to seek out one other dependable process for DNA extraction of putrefied samples relating to tissue choice, prices and time, two industrial forensic kits have been in contrast: DNeasy® Blood & Tissue equipment and SwabSolution™ equipment.
Both kits have been used for DNA extraction from 5 delicate tissues (mind, aorta, liver, kidney and psoas main muscle) and nails (finger- and toenail) obtained through the medicolegal post-mortem of 20 putrefied corpses. DNeasy® Blood & Tissue equipment was by quantitative comparability principally superior to SwabSolution™ equipment: it yielded extra DNA of higher high quality (i.e. much less degraded and inhibited). However, the qualitative outcomes (DNA profiles) of each extraction procedures have been comparable. Among the analyzed tissue sorts, nails have been proved as probably the most appropriate for DNA-based identification of putrefied bodies.
DNA phosphorothioate (PT) modification is a novel modification that happens on the DNA spine, which refers to a non-bridging phosphate oxygen changed by sulfur. This unique DNA modification broadly distributes in micro organism however has not been discovered in eukaryotes up to now. PT modification renders DNA nuclease tolerance and serves as a represent ingredient of bacterial restriction-modification (R-M) defensive system and extra organic features are awaiting exploration. Identification and quantification of the bacterial PT modifications are thus crucial to raised understanding their organic features.
This work describes three detailed strategies derived from iodine-induced particular cleavage-an iodine-induced cleavage assay (ICA), a deep sequencing of iodine-induced cleavage at PT web site (ICDS) and an iodine-induced cleavage PT sequencing (PT-IC-Seq)-for the investigation of PT modifications. Using these approaches, we’ve recognized the presence of PT modifications and quantized the frequency of PT modifications in micro organism. These characterizations contributed to the high-resolution genomic mapping of PT modifications, in which the distribution of PT modification websites on the genome was marked precisely and the frequency of the precise modified websites was reliably obtained.
Identification of Novel Interspersed DNA Repetitive Elements in the Trypanosoma cruzi Genome Associated with the three’UTRs of Surface Multigenic Families
Trypanosoma cruzi is the etiological agent of Chagas illness, which impacts thousands and thousands of folks in Latin America. No transcriptional management of gene expression has been demonstrated in this organism, and 50% of its genome consists of repetitive components and members of multigenic households. In this research, we utilized a novel bioinformatics method to foretell new repetitive components in the genome sequence of T. cruzi. A brand new repetitive sequence measuring 241 nt was recognized and located to be interspersed alongside the genome sequence from strains of completely different DTUs.
This new repeat was totally on intergenic areas, and upstream and downstream areas of the 241 nt repeat have been enriched in floor protein genes. RNAseq analysis revealed that the repeat was half of processed mRNAs and was predominantly discovered in the three’ untranslated areas (UTRs) of genes of multigenic households encoding floor proteins. Moreover, we detected a correlation between the presence of the repeat in the three’UTR of multigenic household genes and the extent of differential expression of these genes when evaluating epimastigote and trypomastigote transcriptomes. These knowledge recommend that this sequence performs a job in the posttranscriptional regulation of the expression of multigenic households.
Mosquitoes are generally recognized to species degree utilizing morphological traits, however complementary strategies for identification are sometimes vital when specimens are collected as immature phases, saved inadequately or when delineation of species complexes is problematic. DNA-barcoding utilizing the mitochondrial cytochrome c oxidase subunit 1 (COI) gene is one such device used for the morphological identification of species. A complete entomological survey of mosquito species in Mexico State recognized by COI DNA barcoding and morphology is documented in this paper.
Specimens have been collected from all of the physiographic provinces in Mexico State between 2017 and 2019. Overall, 2,218 specimens have been collected from 157 localities representing each subfamilies Anophelinae and Culicinae. A species guidelines that consists of 6 tribes, 10 genera, 20 subgenera, and 51 species, 35 of that are new information for Mexico State, is offered. Three hundred and forty-two COI sequences of 46 species have been analysed. Mean intraspecific and interspecific distances ranged between 0% to three.9% and from 1.2% to 25.3%, respectively.
All species teams have been supported by excessive bootstraps values in a Neighbour-Joining analysis and new COI sequences have been generated for eight species: Aedes chionotum Zavortink, Ae. vargasi Schick, Ae. gabriel Schick, Ae. guerrero Berlin, Ae. ramirezi Vargas and Downs, Haemagogus mesodentatus Komp and Kumm, Culex restrictor Dyar and Knab, and Uranotaenia geometrica Theobald. This research gives an in depth stock of the Culicidae from Mexico State and discusses the utility of DNA barcoding as a complementary device for correct mosquito species identification in Mexico.
Identification of fetal unmodified and 5-hydroxymethylated CG websites in maternal cell-free DNA for non-invasive prenatal testing
Massively parallel sequencing of maternal cell-free DNA (cfDNA) is broadly used to check fetal genetic abnormalities in non-invasive prenatal testing (NIPT). However, sequencing-based approaches are nonetheless of excessive price. Building upon earlier data that placenta, the principle supply of fetal circulating DNA, is hypomethylated in comparability to maternal tissue counterparts of cfDNA, we suggest that focusing on both unmodified or 5-hydroxymethylated CG websites particularly enriches fetal genetic materials and reduces numbers of required analytical sequencing reads thereby lowering price of a take a look at.
We employed uTOPseq and hmTOP-seq approaches which mix covalent derivatization of unmodified or hydroxymethylated CG websites, respectively, with subsequent era sequencing, or quantitative real-time PCR. We detected elevated 5-hydroxymethylcytosine (5hmC) ranges in fetal chorionic villi (CV) tissue samples as in contrast with peripheral blood. Down syndrome-specific differentially modified and 5-hydroxymethylated areas, respectively, that can be utilized as biomarkers for identification of Down syndrome or different epigenetic illnesses of a fetus.
 Human Genomic DNA |
|
X11000 |
EpiGentek |
0.2 ml |
Ask for price |
|
Description: reagents widely cited |
 Plant Genomic DNA Kit |
|
20-abx098077 |
Abbexa |
|
|
|
|
 Bacteria Genomic DNA Kit |
|
20-abx098080 |
Abbexa |
|
|
|
|
 Micro Genomic DNA Kit |
|
20-abx098242 |
Abbexa |
|
|
|
|
 Blood Genomic DNA Kit |
|
abx098868-50rxns |
Abbexa |
50 rxns |
EUR 272 |
|
|
Plant Genomic DNA Kit |
|
abx294004-50preps |
Abbexa |
50 preps |
EUR 398 |
|
|
 Animal Genomic DNA Kit |
|
abx294005-100preps |
Abbexa |
100 preps |
EUR 523 |
|
|
Animal Genomic DNA Kit |
|
abx294005-50preps |
Abbexa |
50 preps |
EUR 398 |
|
|
 Human Brain Genomic DNA |
|
X11001 |
EpiGentek |
10 µg |
Ask for price |
|
Description: kits suitable for this type of research |
 Genomic DNA Isolation Kit |
|
K2118-50 |
ApexBio |
50 assays |
EUR 321 |
 Soil Genomic DNA Kit |
|
K1411-250 |
Biovision |
|
EUR 561 |
Soil Genomic DNA Kit |
|
K1411-50 |
Biovision |
|
EUR 316 |
 Insect Genomic DNA Kit |
|
K1412-250 |
Biovision |
|
EUR 561 |
Insect Genomic DNA Kit |
|
K1412-50 |
Biovision |
|
EUR 316 |
 Mollusc Genomic DNA Kit |
|
K1413-250 |
Biovision |
|
EUR 561 |
Mollusc Genomic DNA Kit |
|
K1413-50 |
Biovision |
|
EUR 316 |
 Yeast Genomic DNA Kit |
|
K1414-250 |
Biovision |
|
EUR 561 |
Yeast Genomic DNA Kit |
|
K1414-50 |
Biovision |
|
EUR 316 |
 Fungal Genomic DNA Kit |
|
K1415-250 |
Biovision |
|
EUR 561 |
Fungal Genomic DNA Kit |
|
K1415-50 |
Biovision |
|
EUR 316 |
Genomic DNA Isolation Kit |
|
K281-50 |
Biovision |
|
EUR 272 |
Genomic DNA Isolation Kit |
|
55R-1362 |
Fitzgerald |
50 assays |
EUR 385 |
|
Description: Genomic DNA Isolation Kit for use in the research laboratory |
 Genomic DNA - Human Male |
|
D1234999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Human Female |
|
D1234999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Mouse Male |
|
D1334999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Mouse Female |
|
D1334999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Rat Male |
|
D1434999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Rat Female |
|
D1434999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Dog Male |
|
D1734999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Dog Female |
|
D1734999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Rabbit Male |
|
D1834999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Rabbit Female |
|
D1834999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Porcine Male |
|
D1934999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Porcine Female |
|
D1934999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Bovine Male |
|
D1B34999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Bovine Female |
|
D1B34999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Chicken Male |
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D1C34999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Chicken Female |
|
D1C34999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Hamster Male |
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D1H34999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Hamster Female |
|
D1H34999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Horse Male |
|
D1O34999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Horse Female |
|
D1O34999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA Extraction Kit |
|
K5016005 |
Biochain |
1 kit |
EUR 329 |
 Genomic DNA from Human Adult Normal Tissue: Placenta, from a single donor |
|
D1234200 |
Biochain |
100 ug |
EUR 282 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Guinea pig Placenta Cadherin ELISA kit |
|
E05P0016-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
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Description: A sandwich ELISA for quantitative measurement of Guinea pig Placenta Cadherin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Placenta Cadherin ELISA kit |
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E05P0016-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
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Description: A sandwich ELISA for quantitative measurement of Guinea pig Placenta Cadherin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Placenta Cadherin ELISA kit |
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E05P0016-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A sandwich ELISA for quantitative measurement of Guinea pig Placenta Cadherin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Guinea pig Placenta Growth Factor ELISA kit |
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E05P0018-192T |
BlueGene |
192 tests |
EUR 1270 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Guinea pig Placenta Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Placenta Growth Factor ELISA kit |
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E05P0018-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Guinea pig Placenta Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Placenta Growth Factor ELISA kit |
|
E05P0018-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Guinea pig Placenta Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Plant Genomic DNA extraction buffer |
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20-abx098075 |
Abbexa |
|
|
|
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 Marine Animal Genomic DNA Kit |
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20-abx098079 |
Abbexa |
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 FFPE Tissue Genomic DNA Kit |
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abx098866-50rxns |
Abbexa |
50 rxns |
EUR 272 |
|
|
 Genomic DNA Extraction and Purification |
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EP10012 |
Neuromics |
100 Tests |
EUR 505 |
 Genomic DNA Extraction KitAnimal Tissue |
|
FYG102-100P |
Yeastern Biotech |
100 Preps |
Ask for price |
Genomic DNA Extraction KitAnimal Tissue |
|
FYG102-300P |
Yeastern Biotech |
300 Preps |
Ask for price |
Genomic DNA Extraction KitAnimal Tissue |
|
FYG102-50P |
Yeastern Biotech |
50 Preps |
Ask for price |
 Genomic DNA Extraction Maxi KitPlant |
|
FYG104-10P |
Yeastern Biotech |
10 Preps |
Ask for price |
Genomic DNA Extraction Maxi KitPlant |
|
FYG104-25P |
Yeastern Biotech |
25 Preps |
Ask for price |
 Zenoquick Genomic DNA Purification Kit |
|
Z2003-200 |
GenDepot |
200 preps |
EUR 631 |
 Zenoprep Genomic DNA Purification Kit |
|
Z2004-400 |
GenDepot |
400 preps |
EUR 550 |
 ISOLATE II Genomic DNA Kit |
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BIO-52065 |
Bioline |
10 preps |
Ask for price |
ISOLATE II Genomic DNA Kit |
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BIO-52066 |
Bioline |
50 preps |
Ask for price |
ISOLATE II Genomic DNA Kit |
|
BIO-52067 |
Bioline |
250 preps |
Ask for price |
 AccuRT Genomic DNA Removal Kit |
|
G488 |
ABM |
200 Reactions |
EUR 82 |
 Bacterial Genomic DNA Isolation Kit |
|
K309-100 |
Biovision |
|
EUR 376 |
 Genomic DNA - Cynomolgus Monkey Male |
|
D1534999-Cy-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Cynomolgus Monkey Female |
|
D1534999-Cy-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Rhesus Monkey Male |
|
D1534999-G01 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 Genomic DNA - Rhesus Monkey Female |
|
D1534999-G02 |
Biochain |
100 ug |
EUR 188 |
|
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
 100 BP DNA LADDER, 500UL PER KIT |
|
M-DNA-100BP |
CORNING |
1/pk |
EUR 73 |
|
Description: Bioscience Mol Bio; DNA Ladder |
 1 KB DNA LADDER, 500UL PER KIT |
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M-DNA-1KB |
CORNING |
1/pk |
EUR 70 |
|
Description: Bioscience Mol Bio; DNA Ladder |
 ELISA Kit) Guinea pig Placenta Growth Factor (PGF) ELISA Kit |
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abx357827-96tests |
Abbexa |
96 tests |
EUR 825 |
|
|
 Rapid Fungal Genomic DNA Isolation Kit |
|
FT71415 |
Bio Basic |
50Preps |
EUR 76.64 |
|
|
 Rapid Animal Genomic DNA Extraction Kit |
|
AT4780 |
Bio Basic |
10Preps |
EUR 69.03 |
|
|
Rapid Animal Genomic DNA Extraction Kit |
|
AT4781 |
Bio Basic |
50Preps |
EUR 126.13 |
|
|
Rapid Animal Genomic DNA Extraction Kit |
|
AT4782 |
Bio Basic |
250preps |
EUR 354.5 |
|
|
 Rapid Bacteria Genomic DNA Isolation Kit |
|
BS8225 |
Bio Basic |
50Preps |
EUR 76.64 |
|
|
 Rapid Yeast Genomic DNA Extraction Kit |
|
BS8227 |
Bio Basic |
50Preps |
EUR 76.64 |
|
|
 Rapid Blood Genomic DNA Extraction Kit |
|
BT4782 |
Bio Basic |
50Preps |
EUR 76.64 |
|
|
) Genomic DNA Kit (No RNase A) |
|
20-abx098862 |
Abbexa |
|
|
|
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 Genomic DNA Extraction Mini KitPlant Tissue |
|
FYG103-100P |
Yeastern Biotech |
100 Preps |
Ask for price |
 Easy3-Plant Genomic DNA Isolation Kit |
|
FYG503-100P |
Yeastern Biotech |
100 Preps |
Ask for price |
Easy3-Plant Genomic DNA Isolation Kit |
|
FYG503-500P |
Yeastern Biotech |
500 Preps |
Ask for price |
 Fully CpG Methylated Human Genomic DNA |
|
X10000 |
EpiGentek |
10 µg/100 µl |
Ask for price |
|
Description: The best epigenetics products |
 Plant Tissue Genomic DNA Isolation Kit |
|
K316-100 |
Biovision |
|
EUR 376 |
 Mammalian Cell Genomic DNA Isolation Kit |
|
K967-100 |
Biovision |
|
EUR 381 |
 Guinea Pig Anti-DNA ELISA Kit |
|
EGD0216 |
Abclonal |
96Tests |
EUR 521 |
) Blood Genomic DNA Extraction Mini Kit (100prep) |
|
FABGK-100 |
Favorgen |
100 preps |
EUR 165 |
) Blood Genomic DNA Extraction Mini Kit (300prep) |
|
FABGK-300 |
Favorgen |
300 preps |
EUR 266 |
) FavorPrep Genomic DNA Clean-Up Kit (50prep) |
|
FAGDC-001 |
Favorgen |
50 preps |
EUR 129 |
) FavorPrep Genomic DNA Clean-Up Kit (200prep) |
|
FAGDC-001-1 |
Favorgen |
200 preps |
EUR 204 |
) Plant Genomic DNA Extraction Mini Kit (50prep) |
|
FAPGK-001 |
Favorgen |
50 preps |
EUR 158 |
) Plant Genomic DNA Extraction Mini Kit (100prep) |
|
FAPGK-001-1 |
Favorgen |
100 preps |
EUR 204 |
) Plant Genomic DNA Extraction Mini Kit (200prep) |
|
FAPGK-001-2 |
Favorgen |
200 preps |
EUR 274 |
) Plant Genomic DNA Extraction Maxi Kit (10prep) |
|
FAPGK-002 |
Favorgen |
10 preps |
EUR 201 |
Using our beforehand developed uTOP-seq and hmTOP-seq approaches we obtained whole-genome uCG and 5hmCG maps of 10 CV tissue and 38 cfDNA samples in complete. Our outcomes indicated that, in distinction to traditional complete genome sequencing, such epigenomic analysis extremely particularly enriches fetal DNA fragments from maternal cfDNA. While each our approaches yielded 100% accuracy in detecting Down syndrome in fetuses, hmTOP-seq maintained such accuracy at ultra-low sequencing depths utilizing just one million reads. We recognized 2164 and 1589 placenta-specific differentially modified and 5-hydroxymethylated areas, respectively, in chromosome 21, in addition to 3490 and 2002
