Month: March 2021

Novel and Efficient Protocol for DNA Coating-Based Identification of DNA-Protein Interaction by Antibody-Mediated Immunodetection

Novel and Efficient Protocol for DNA Coating-Based Identification of DNA-Protein Interaction by Antibody-Mediated Immunodetection

Studying protein-protein and protein-DNA interactions are conditions for the identification of operate and mechanistic function of numerous proteins within the cell. Protocols for analyzing DNA-based Protein-Protein and Protein-DNA interactions are difficult and must be simplified for environment friendly monitoring of binding capabilities of numerous proteins to particular DNA molecules. Here, we demonstrated a easy but …

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From 18S to 28S rRNA Gene: An Improved Targeted Sarcocystidae PCR Amplification, Species Identification with Long DNA Sequences

From 18S to 28S rRNA Gene: An Improved Targeted Sarcocystidae PCR Amplification, Species Identification with Long DNA Sequences

Sarcocystosis outbreaks in Tioman and Pangkor islands of Malaysia between 2011 and 2014 have raised the necessity to enhance Sarcocystis species detection from environmental samples. In-house works discovered that revealed primers amplifying the 18S rRNA gene of Sarcocystis both couldn’t produce the goal from environmental samples or produced Sarcocystis DNA sequence that was inadequate for …

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Identification of the ternary complex of ribonuclease HI:RNA/DNA hybrid:metal ions by ESI mass spectrometry

Identification of the ternary complex of ribonuclease HI:RNA/DNA hybrid:metal ions by ESI mass spectrometry

Ribonuclease HI, an endoribonuclease, catalyzes the hydrolysis of the RNA strand of an RNA/DNA hybrid, and requires divalent metallic ions for its enzymatic exercise. However, the mechanistic particulars of the exercise of ribonuclease HI and its interplay with divalent metallic ions stays unclear. In this examine, we carried out real-time monitoring of the enzyme-substrate complex …

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